Gene description for ACTN4
Gene name actinin alpha 4
Gene symbol ACTN4
Other names/aliases ACTININ-4
FSGS
FSGS1
Species Homo sapiens
 Experiment description of studies that identified ACTN4 in Breast cancer cells

1
Experiment ID575
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Mass spectrometry [LTQ]
PubMed ID 27894104   
OrganismHomo sapiens
Experiment descriptionProteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.
AuthorsHurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr.
Journal name Oncotarget
Publication year2016
SampleBreast cancer cells
Sample nameBT549
Isolation/purification methodsDifferential centrifugation
Polymer-based precipitation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry [LTQ]
EV-TRACK -

2
Experiment ID1028
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 26378940   
OrganismHomo sapiens
Experiment descriptionRedefining the Breast Cancer Exosome Proteome by Tandem Mass Tag Quantitative Proteomics and Multivariate Cluster Analysis.
AuthorsClark DJ, Fondrie WE, Liao Z, Hanson PI, Fulton A, Mao L, Yang AJ.
Journal name Anal Chem.
Publication year2015
SampleBreast cancer cells
Sample nameSKBR3B - 10 K pellet
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK EV150004: EV-METRIC:67%, 38%

3
Experiment ID2452
Identified moleculeprotein
Extracellular vesicle typeApoptotic bodies
MISEVLarge EVs
Identification method Western blotting
PubMed ID 34465793   
OrganismHomo sapiens
Experiment descriptionExosomal miR-19a and IBSP cooperate to induce osteolytic bone metastasis of estrogen receptor-positive breast cancer
AuthorsWu K, Feng J, Lyu F, Xing F, Sharma S, Liu Y, Wu SY, Zhao D, Tyagi A, Deshpande RP, Pei X, Ruiz MG, Takahashi H, Tsuzuki S, Kimura T, Mo YY, Shiozawa Y, Singh R, Watabe K.
Journal name Nat Commun
Publication year2021
SampleBreast cancer cells
Sample nameMCF7BoM2 - 2K pellet
Isolation/purification methodsCentrifugation
Flotation density-
Molecules identified in the studyProtein
miRNA
Methods used in the studyWestern blotting
qPCR
EV-TRACK -

4
Experiment ID2453
Identified moleculeprotein
Extracellular vesicle typeMicrovesicles
MISEVLarge EVs
Identification method Western blotting
PubMed ID 34465793   
OrganismHomo sapiens
Experiment descriptionExosomal miR-19a and IBSP cooperate to induce osteolytic bone metastasis of estrogen receptor-positive breast cancer
AuthorsWu K, Feng J, Lyu F, Xing F, Sharma S, Liu Y, Wu SY, Zhao D, Tyagi A, Deshpande RP, Pei X, Ruiz MG, Takahashi H, Tsuzuki S, Kimura T, Mo YY, Shiozawa Y, Singh R, Watabe K.
Journal name Nat Commun
Publication year2021
SampleBreast cancer cells
Sample nameMCF7BoM2 - 10K pellet
Isolation/purification methodsCentrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
miRNA
Methods used in the studyWestern blotting
qPCR
EV-TRACK -

5
Experiment ID1664
Identified moleculeProtein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Mass spectrometry
PubMed ID 34108659   
OrganismHomo sapiens
Experiment descriptionQuantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
AuthorsKugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R.
Journal name Nat Cell Biol
Publication year2021
SampleBreast cancer cells
Sample nameMCF7
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
mRNA
Methods used in the studyRT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
EV-TRACK -

6
Experiment ID1666
Identified moleculeProtein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Mass spectrometry
PubMed ID 34108659   
OrganismHomo sapiens
Experiment descriptionQuantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
AuthorsKugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R.
Journal name Nat Cell Biol
Publication year2021
SampleBreast cancer cells
Sample nameMDA-MB-231
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
mRNA
Methods used in the studyRT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
EV-TRACK -

7
Experiment ID1678
Identified moleculeProtein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Mass spectrometry
PubMed ID 34108659   
OrganismHomo sapiens
Experiment descriptionQuantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
AuthorsKugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R.
Journal name Nat Cell Biol
Publication year2021
SampleBreast cancer cells
Sample nameMDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
Mass spectrometry
EV-TRACK -

8
Experiment ID1679
Identified moleculeProtein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Mass spectrometry
PubMed ID 34108659   
OrganismHomo sapiens
Experiment descriptionQuantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
AuthorsKugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R.
Journal name Nat Cell Biol
Publication year2021
SampleBreast cancer cells
Sample nameMDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -

9
Experiment ID1759
Identified moleculeProtein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Mass spectrometry
PubMed ID 35918900   
OrganismHomo sapiens
Experiment descriptionCharacterization of protein complexes in extracellular vesicles by intact extracellular vesicle crosslinking mass spectrometry (iEVXL)
AuthorsBauzá-Martinez J, Armony G, Pronker MF, Wu W.
Journal name J Extracell Vesicles
Publication year2022
SampleBreast cancer cells
Sample nameLM2
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectometry
EV-TRACK -

10
Experiment ID1761
Identified moleculeProtein
Extracellular vesicle typeExtracellular vesicles
MISEVSmall EVs
Identification method Mass spectrometry
PubMed ID 35918900   
OrganismHomo sapiens
Experiment descriptionCharacterization of protein complexes in extracellular vesicles by intact extracellular vesicle crosslinking mass spectrometry (iEVXL)
AuthorsBauzá-Martinez J, Armony G, Pronker MF, Wu W.
Journal name J Extracell Vesicles
Publication year2022
SampleBreast cancer cells
Sample nameLM2
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectometry
EV-TRACK -

11
Experiment ID3350
Identified moleculeprotein
Extracellular vesicle typeSupermeres
MISEVEV-like nanoparticles
Identification method Mass spectrometry
PubMed ID 34887515   
OrganismHomo sapiens
Experiment descriptionSupermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
AuthorsZhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ.
Journal name Nat Cell Biol
Publication year2021
SampleBreast cancer cells
Sample nameMDA-MB-231
Isolation/purification methodsDifferential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
Mass spectrometry
ELISA
EV-TRACK -

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